Isolated dc-dc charger

Can I use the ground points in my driver’s seat base on my 2016 sprinter, instead of connecting to the negative post on the battery?

Yes, but make sure that the ground point is in good electrical and mechanical condition, clean metal with good contact. Using the chassis ground for Orion chargers is one of the most common reasons for them not working well because the connection has poor electrical contact. Direct battery connections often have better conductivity.